Hideyuki TANABE Associate Professor

E-mail:
ResearchMap: http://researchmap.jp/hayama_nanafushi/

Molecular Cytogenetics, Evolutionary Studies of Chromosomes and Genomics

The genomes of eukaryotes are folded into the structure “chromosome territories”, we can see the whole genomes of each species by observing the chromosomes. I am focused on the interphase chromosomes and how those chromosomes are spatially organized into the cell nucleus and what kind of regulatory factors are involved in the spatial positioning of chromosomes and genes by molecular cytogenetic approaches such as FISH (Fluorescence In Situ Hybridization) techniques. The interphase chromosomes are occupied within the cell nucleus as “chromosome territories” that are highly compartmentalized, mutually exclusive, and radially positioned in correlation with their physical sizes and gene densities. It is known that several dynamic spatial movements of chromosome territories and/or genes during embryogenesis, cellular differentiation, changes of physiological environment, tumorigenesis, and ageing in addition to that chromosomal translocations are due to their spatially relative chromosome positioning during the course of evolution. However, the molecular mechanisms are still not yet understood.

Then I am studying by the following techniques, multi-color FISH (Fluorescence In Situ Hybridzation), 3D-FISH, 3C (Chromosome Conformation Capture), microdissection for chromosome spreads, and genome editing for analyses of chromosomal evolution and spatial organization of chromosome territories and genes within the cell nucleus using cultured cell lines derived from humans, primates, mammals, and birds including primary cultured cells, tumor cells, stem cells, and embryos.

hideyuki_tanabe

Papers and publications

  1. R. Kawamura, H. Tanabe, T. Wada, S. Saitoh, Y. Fukushima and K. Wakui* (2012). Visualization of the spatial positioning of the SNRPN, UBE3A, and GABRB3 genes in the normal human nucleus by three-color 3D fluorescence in situ hybridization. Chromosome Research 20, 659-672.
  2. E. Ohfuchi-Maruyama, T. Hori, H. Tanabe*, H. Kitamura, R. Matsuda, S. Tone, P. Hozak, F. A. Habermann, J. von Hase, C. Cremer, T. Fukagawa and M. Harata* (2012). The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei. Journal of Cell Science 125, 3739-3743.
  3. T. Amano, T. Sagai, H. Tanabe, Y. Mizushina, H. Nakazawa and T. Shiroishi* (2009). Chromosomal dynamics at the Shh locus: Lim bud-specific differential regulation of competence and active transcription. Developmental Cell 16, 47-57.
  4. H. Tanabe*, K. Küpper, T. Ishida, M. Neusser and H. Mizusawa (2005). Inter- and intra-specific gene-density correlated radial chromosome territory arrangements are conserved in Old World monkeys. Cytogenetics and Genome Research 108, 255-261.
  5. H. Tanabe, S. Müller, M. Neusser, J. von Hase, E. Calcagno, M. Cremer, I. Solovei, C. Cremer and T. Cremer* (2002). Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates. Proceedings of the National Academy of Sciences USA 99, 4424-4429.
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