About

Communal Microscope Facilities

ESB has a transmission electron microscope, scanning electron microscope, two confocal laser-scanning microscopes, and a high-speed camera. The facility is available for use by all qualified faculty, students, and researchers at SOKENDAI, as well as collaborators from other institutes.

Download:
terms & conditions pdf(xxkb)
application form word(xxKB), pdf(xxKB)
For detailed information, please contact:
Atsuko Matsushita []

1Transmission Electron Microscope

We support conventional EM and immunocytochemistry from tissue preparation to image acquisition and analysis.
Specimen examples: insect compound eyes, CNS of mammals and fish, and reproductive organ of mice
Location: Sendo-ken bldg. Room 120

  • Transmission electron microscope(Hitachi H-7650)
  • Two Digital CCD cameras (Seika Digital Image Corp.)
    • Morada: 11MegaPixel Side-mounted CCD camera (Max. 2672 x 4008 pixel)
    • Quemesa: 11MegaPixel Bottom-mounted CCD camera (Max. 4008 x 2664 pixel)
    • imaging software: iTEM (image acquisition, processing, morphometry, etc.)
    • Common file formats for exporting image data
  • Ultramicrotome (Leica UCT)
  • The facility is also equipped with a light microscope (Olympus BX51 (Brightfield/ fluorescence/ Nomarski DIC) with a digital camera (Olympus DP71, software cellSens), and a PC for iTEM analysis.

Transmission Electron Microscope equipped with a CCD camera (Hitachi H-7650 and Morada)

Installed in 2006

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Ultramicrotome (Leica UCT)

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2Scanning Electron Microscope

We support whole general protocol from specimen preparation to image scanning.
Specimen examples: insects, archaeological animal/plant remains and their replicas, tips of glass electrodes
Location: Sendo-ken bldg. Room 118

  • Scanning Electron Microscope (JEOL JSM-6490LV)
    • Secondary-electron and backscatter-electron images at High-Vacuum mode
    • Backscatter-electron images at Low-Vacuum mode
    • Analyses with topography image and composition images at backscatter-electron detection mode
    • Large specimen (< 85 mm) available
    • Common file formats for saving/exporting files
  • Ion Sputter Coater(Hitachi E-1045)
    • Coats those non-conductive specimens with platinum sputtering or carbon evaporation to prevent charge-up under SEM
  • Freeze Dryer(Hitachi ES-2030)
    • Removes water from wet specimen by sublimation under frozen-vacuumed condition, preventing specimen distortion under SEM

Scanning Electron Microscope (JEOL JSM-6490LV)

Installed in 2008

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Ion Sputter Coater(Hitachi E-1045)

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Freeze Dryer(Hitachi ES-2030)

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3Confocal Laser Scanning Microscope (Zeiss)

We support the use of this confocal laser-scanning microscope, especially for 3D-image acquisitions of nuclear architecture, chromosome territories, genes, and other molecules within cell nuclei by multi-color visualization techniques.

Specimen examples: Chromosomes from mouse tissue, HeLa cells, and human lymphocyte cell lines
Location: Sendo-ken bldg. Room 211

  • Confocal laser scanning microscope(Carl Zeiss LSM510META)
    • Laser sources (fine-tuned by AOTF)
      1. Ar458/477/488/514nm
      2. HeNe543nm
      3. HeNe633nm
    • META multi (32) channel detector & high-sensitive PMT detector
    • High-speed acquisition of Lambda Stack function, Metatracking scanning mode, and Emission Fingerprinting function.
    • ROI (region of interest) scanning for specimen protection
    • Photo-bleaching analyses such as FRAP and FLIP are available.
    • Stack Images are saved in Carl Zeiss LSM browser (LSM5) format; Common file formats for exporting files
    • ReUse function: 1-click to replicate parameters for image acquisition

Confocal Laser Scanning Microscope(Carl Zeiss LSM510META)

Installed in 2004

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4Confocal laser scanning microscope system (Nikon)

We provide technical supports for various imaging such as multicolor/DIC imaging of either 2D or 3D, simultaneous acquisition of 32-ch spectral images, time-lapse imaging, and photoactivation imaging, by using Nikon A1Rsi configured with Ti-E.
Specimen examples: Neurons of various species, such as insects, echinoderms, and fish; developing echinoderm embryos and fish embryos; photoactivation imaging of algae
Location: Sendo-ken bldg. Room 218

  • Confocal laser scanning microscope (Nikon Ti-E & A1Rsi)
    • Laser sources (fine-tuned by AOTF):
      1. LD 405 nm
      2. multi-Ar 457/477/488/514 nm
      3. DPSS 561 nm
      4. LD 638 nm
    • Inverted light microscope (ECLIPSE Ti-E); motorized stage and fluorescence filters, "Perfect focus system" for automatically correcting focus drift
    • High-resolution with a galvano scanner (Max. 4096 x 4096 pixels),
    • High-speed with a resonant scanner (512 x 512 pixels, 24 frame/sec),
    • Hybrid scanner for ultrafast photoactivation imaging
    • Automatic Enhanced Resolution imaging (NIS-Elements C-ER): Higher resolution images by just 1-click operation (Upgraded in March 2016)
    • Common file formats (avi, tiff, jpg) are available as well as nd2.
  • The facility is also equipped with a stereoscope for sample preparation, incubator (with light/temperature controller) for temporal incubation, and PC for image analysis.
  • Light microscope (Olympus BX51, fluorescence/Nomarski DIC/phase contrast) with a digital camera (Olympus DP72)
    - Available for preliminary check of the sample. (in the first room of Room 218)

Confocal laser scanning microscope (Nikon Ti-E & A1Rsi)

Installed in 2010

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Light microscope (Olympus BX51, with DP72)

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5High-speed camera (Photoron FASTCAM-Max II)

Recorded example: a flying butterfly
Location: Sendo-ken bldg. Room 118. All devices are housed in one cart, it is available everywhere to go within the campus.

High-speed camera (Photoron FASTCAM-MAX, FASTCAM-MAX II)

Installed in 2010

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  1. Two camera heads:
  2. Tripod
  3. Memory: Standard 2.6GB (1sec, at full size & 2000fps), Max 8GB (3.1sec), connectable to PC and a monitor
  4. PC for camera controller, data storing: Windows XP, data transfer by optical cable transmission system
  5. monitor, keyboard, and mouse
FASTCAM-MAX
Color imaging
FASTCAM-MAX II
High-sensitive sensor, for low light condition
Sensor C-MOS image sensors
Max resolution 1024 x 1024 pixel, color 1024 x 1024 pixel, monochrome
Frame rate 2000 frame/sec (fullframe)
Camera mount C-mount and Nikon F-mount, available for lens and microscopes